Lamp1 monoclonal antibody, clone 1D4B (APC)
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Specification
Product Description
Rat monoclonal antibody raised against native Lamp1.
Immunogen
Native purified Lamp1 from plasma membrane fraction of mouse embryo NIH3T3 cell line.
Host
Rat
Reactivity
Mouse
Specificity
Murine CD107a/LAMP-1 (Mr 110-140 KDa with a core protein of Mr 40 KDa).
Form
Liquid
Conjugation
APC
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
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Gene Info — Lamp1
Entrez GeneID
16783Gene Name
Lamp1
Gene Alias
AI196048, CD107a, Lamp-1
Gene Description
lysosomal-associated membrane protein 1
Gene Ontology
HyperlinkOther Designations
LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B)|Lamp I|lysosomal membrane glycoprotein 1
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Publication Reference
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The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane.
Rohrer J, Schweizer A, Russell D, Kornfeld S.
The Journal of Cell Biology 1996 Feb; 132(4):565.
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Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking.
Fukuda M.
The Journal of Biological Chemistry 1991 Nov; 266(32):21327.
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The disulfide structure of mouse lysosome-associated membrane protein 1.
Arterburn LM, Earles BJ, August JT.
The Journal of Biological Chemistry 1990 May; 265(13):7419.
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The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane.
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