H2-D4 monoclonal antibody, clone 34-5-8S (Biotin)
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Specification
Product Description
Mouse monoclonal antibody raised against H2-D4.
Immunogen
C57BL/6 x DBA/2)F1 hybrid mouse splenocytes.
Host
Mouse
Reactivity
Mouse
Specificity
A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen.
Form
Liquid
Conjugation
Biotin
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
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Publication Reference
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Peptide and beta 2-microglobulin regulation of cell surface MHC class I conformation and expression.
Otten GR, Bikoff E, Ribaudo RK, Kozlowski S, Margulies DH, Germain RN.
Journal of Immunology 1993 Jul; 148(13):3724.
Application:Flow Cyt, Mouse, LKD8 cells.
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Monoclonal antibodies to mouse major histocompatibility complex antigens.
Ozato K, Mayer NM, Sachs DH.
Transplantation 1982 Sep; 34(3):113.
Application:Flow Cyt, Human, Mammalian cells.
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Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens.
Ozato K, Mayer N, Sachs DH.
Journal of Immunology 1980 Feb; 124(2):533.
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Peptide and beta 2-microglobulin regulation of cell surface MHC class I conformation and expression.
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