Il2rb monoclonal antibody, clone 5H4 (APC)
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More Files
- More Functions
Flow Cytometry
BALB/c lymph node cells were double stained with Cd3e monoclonal antibody, clone C363.29B (PE) (Cat # MAB6102) and Il2rb monoclonal antibody, clone 5H4 (FITC) (Cat # MAB5807). Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Il2rb.
Immunogen
Rat myeloma YB2/0 transfected with truncated mouse IL-2RB cDNA.
Host
Rat
Reactivity
Mouse
Specificity
beta chain (Mr 90-100 KDa) of the IL-2 receptor.
Form
Liquid
Conjugation
APC
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
BALB/c lymph node cells were double stained with Cd3e monoclonal antibody, clone C363.29B (PE) (Cat # MAB6102) and Il2rb monoclonal antibody, clone 5H4 (FITC) (Cat # MAB5807). Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Il2rb
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Publication Reference
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Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits.
Malek TR, Furse RK, Fleming ML, Fadell AJ, He YW.
Journal of Interferon & Cytokine Research: the Official Journal of the International Society for Int 1995 May; 15(5):447.
Application：Flow Cyt, Mouse , Mouse EL4 cells.
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The IL-2 receptor complex: its structure, function, and target genes.
Minami Y, Kono T, Miyazaki T, Taniguchi T.
Annual Review of Immunology 1993 Apr; 11:245.
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A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells.
Tanaka T, Tsudo M, Karasuyama H, Kitamura F, Kono T, Hatakeyama M, Taniguchi T, Miyasaka M.
Journal of Immunology 1991 Oct; 147(7):2222.
Application：Flow Cyt, Mouse, Mouse spleen cells.
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Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits.
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