I-Ek monoclonal antibody, clone 17-3-3S (FITC)
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More Files
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Flow Cytometry
AKR spleen cells were double-stained with I-Ek monoclonal antibody, clone 17-3-3S (FITC) (Cat # MAB5804) and Cd3e monoclonal antibody, clone C363.29B (PE) (Cat # MAB6102). Lymphocytes were gated using and analyzed by flow cytometry.
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Specification
Product Description
Mouse monoclonal antibody raised against I-Ek.
Immunogen
Native purified I-Ek from C3H mouse skin graft and splenocytes.
Host
Mouse
Reactivity
Mouse
Specificity
An epitope on mouse I-Ek MHC class II alloantigen.
Form
Liquid
Conjugation
FITC
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
AKR spleen cells were double-stained with I-Ek monoclonal antibody, clone 17-3-3S (FITC) (Cat # MAB5804) and Cd3e monoclonal antibody, clone C363.29B (PE) (Cat # MAB6102). Lymphocytes were gated using and analyzed by flow cytometry. -
Publication Reference
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Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens.
Ozato K, Mayer N, Sachs DH.
Journal of Immunology 1980 Feb; 124(2):533.
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Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens.
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