Lpam-1 monoclonal antibody, clone DATK32 (PE)
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More Files
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Flow Cytometry
Either the TK-1 cell line (Lpam-1 expressing) or the 18-81 MuLV-transformed pre-B cell line (Lpam-1 negative) was labeled with Lpam-1 monoclonal antibody, clone DATK32 (Cat # MAB3252) and then stained with goat anti-rat IgG-FITC. The primary cell populations on each scatter plot were then gated and analyzed by flow cytometry.
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Specification
Product Description
Rat monoclonal antibody raised against native Lpam-1.
Immunogen
Native purified Lpam-1 from AKR/Cum mouse spontaneous T lymphoma cell line TK1.
Host
Rat
Reactivity
Mouse
Specificity
Mouse LPAM-1 (Integrin alpha4beta7).
Form
Liquid
Conjugation
PE
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Flow Cytometry
Either the TK-1 cell line (Lpam-1 expressing) or the 18-81 MuLV-transformed pre-B cell line (Lpam-1 negative) was labeled with Lpam-1 monoclonal antibody, clone DATK32 (Cat # MAB3252) and then stained with goat anti-rat IgG-FITC. The primary cell populations on each scatter plot were then gated and analyzed by flow cytometry. -
Gene Info — Itga4
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Gene Info — Itgb7
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Publication Reference
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Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes.
Andrew DP, Rott LS, Kilshaw PJ, Butcher EC.
European Journal of Immunology 1996 Apr; 26(4):897.
Application：Flow Cyt, Mouse, Spleen, PP, MLN, Peripheral lymph node cells, Thymocytes, Lymphocytes.
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Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
Andrew DP, Berlin C, Honda S, Yoshino T, Hamann A, Holzmann B, Kilshaw PJ, Butcher EC.
The Journal of Immunology 1994 Nov; 153(9):3847.
Application：Flow Cyt, Mouse, Lymphocytes, Bone marrow cells.
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Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo.
Hamann A, Andrew DP, Jablonski-Westrich D, Holzmann B, Butcher EC.
Journal of Immunology 1994 Apr; 152(7):3282.
Application：Func, Mouse, Mouse lymphocytes.
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Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes.
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