Itga2 monoclonal antibody, clone DX5 (FITC)
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More Files
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Flow Cytometry
Splenocytes from a C57BL/6 SCID mouse were double-stained with Itga2 monoclonal antibody, clone DX5 (FITC) (Cat # MAB5780) and Itga2 monoclonal antibody, clone DX5 (PE) (Cat # MAB5782). Lymphocytes were gated using light scatter criteria and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Itga2.
Immunogen
RIL-2-propagated NK1.1+ cells from C57BL/6 mice.
Host
Rat
Reactivity
Mouse
Specificity
Mouse CD49b, Mr 160-KDa. The DX5 antibody is not suitable for immunohistochemical staining of either frozen or paraffin sections.
Form
Liquid
Conjugation
FITC
Isotype
IgM, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Splenocytes from a C57BL/6 SCID mouse were double-stained with Itga2 monoclonal antibody, clone DX5 (FITC) (Cat # MAB5780) and Itga2 monoclonal antibody, clone DX5 (PE) (Cat # MAB5782). Lymphocytes were gated using light scatter criteria and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Itga2
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Publication Reference
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Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).
Arase H, Saito T, Phillips JH, Lanier LL.
Journal of Immunology 2001 Aug; 167(3):1141.
Application：Flow Cyt, Mouse , Mouse NK cells.
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Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice.
Moore TA, von Freeden-Jeffry U, Murray R, Zlotnik A.
Journal of Immunology 1996 Sep; 157(6):2366.
Application：Flow Cyt, Mouse, Nature killer cells.
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Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).
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