Cd86 monoclonal antibody, clone GL1 (APC)
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More Files
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Flow Cytometry
BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (FITC) (Cat # MAB5751). A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against native Cd86.
Immunogen
Native purified Cd86 from LPS-activated mouse B cells.
Host
Rat
Reactivity
Mouse
Specificity
Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4.
Form
Liquid
Conjugation
APC
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (FITC) (Cat # MAB5751). A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd86
Entrez GeneID
12524Gene Name
Cd86
Gene Alias
B7, B7-2, B7.2, B70, CLS1, Cd28l2, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2
Gene Description
CD86 antigen
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000015940|T-lymphocyte activation antigen CD86|activation B7-2 antigen|early T cell costimulatory molecule-1
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Publication Reference
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The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.
Inaba K, Witmer-Pack M, Inaba M, Hathcock KS, Sakuta H, Azuma M, Yagita H, Okumura K, Linsley PS, Ikehara S, Muramatsu S, Hodes RJ, Steinman RM.
The Journal of Experimental Medicine 1994 Nov; 180(5):1849.
Application：Flow Cyt, Func, IHC-Fr, Mouse, Mouse B cells and dendritic cells, Mouse heart, liver, spleen, thymus, tongue.
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Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.
Hathcock KS, Laszlo G, Pucillo C, Linsley P, Hodes RJ.
The Journal of Experimental Medicine 1994 Aug; 180(2):631.
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Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice.
Freeman GJ, Borriello F, Hodes RJ, Reiser H, Hathcock KS, Laszlo G, McKnight AJ, Kim J, Du L, Lombard DB, et al..
Science 1993 Nov; 262(5135):907.
Application：Flow Cyt, Mouse, Mouse splenic B cells.
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The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.
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