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Last updated: 2016/12/4
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Cd86 monoclonal antibody, clone GL1 (APC)

  • Catalog # : MAB5754
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  • Specification
  • Product Description:
  • Rat monoclonal antibody raised against native Cd86.
  • Immunogen:
  • Native purified Cd86 from LPS-activated mouse B cells.
  • Host:
  • Rat
  • Reactivity:
  • Mouse
  • Specificity:
  • Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4.
  • Form:
  • Liquid
  • Conjugation:
  • APC
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • Flow Cytometry (0.1 ug/106 cells)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Frozen sections)
  • Immunoprecipitation
  • Flow Cytometry
  • Flow Cytometry
  • BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with Cd86 monoclonal antibody, clone GL1 (FITC) (Cat # MAB5751). A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
  • Application Image
  • Immunohistochemistry (Frozen sections)
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • Cd86
  • Gene Alias:
  • B7,B7-2,B7.2,B70,CLS1,Cd28l2,ETC-1,Ly-58,Ly58,MB7,MB7-2,TS/A-2
  • Gene Description:
  • CD86 antigen
  • Other Designations:
  • OTTMUSP00000015940,T-lymphocyte activation antigen CD86,activation B7-2 antigen,early T cell costimulatory molecule-1
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