Cd72 monoclonal antibody, clone 10-1.D2 (Biotin)
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More Files
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Flow Cytometry
BALB/c spleen cells were double-stained with Cd72.1 monoclonal antibody, clone 10-1.D2 (FITC) (Cat # MAB5748) and rat anti-mouse CD19-R-PE. Lymphocytes were then gated and analyzed by flow cytometry.
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Specification
Product Description
Mouse monoclonal antibody raised against native Cd72.
Immunogen
Native purified Cd72 from DBA/2 mouse spleen cells.
Host
Mouse
Reactivity
Mouse
Specificity
Mouse CD72.1/Lyb-2.1, 45 KDa.
Form
Liquid
Conjugation
Biotin
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
BALB/c spleen cells were double-stained with Cd72.1 monoclonal antibody, clone 10-1.D2 (FITC) (Cat # MAB5748) and rat anti-mouse CD19-R-PE. Lymphocytes were then gated and analyzed by flow cytometry. -
Gene Info — Cd72
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Publication Reference
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Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72.
Robinson WH, Landolfi MM, Schafer H, Parnes JR.
Journal of Immunology 1993 Nov; 151(9):4764.
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Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72).
Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K.
Journal of Immunology 1992 Mar; 148(6):1630.
Application：Func, Human, EL4 cells, Lymphocytes.
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Alpha-helical coiled-coil stalks in the low-affinity receptor for IgE (Fc epsilon RII/CD23) and related C-type lectins.
Beavil AJ, Edmeades RL, Gould HJ, Sutton BJ.
PNAS 1992 Jan; 89(2):753.
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Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72.
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