Cd69 monoclonal antibody, clone H1.2F3 (Biotin)
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More Files
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Flow Cytometry
Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or Cd69 monoclonal antibody, clone H1.2F3 (FITC) (Cat # MAB5742). Large, activated cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Hamster monoclonal antibody raised against native Cd69.
Immunogen
Native purified Cd69 from Y245 mouse dendritic epidermal T cell line.
Host
Hamster
Reactivity
Mouse
Specificity
Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 KDa (unreduced).
Form
Liquid
Conjugation
Biotin
Isotype
IgG, lambda
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or Cd69 monoclonal antibody, clone H1.2F3 (FITC) (Cat # MAB5742). Large, activated cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd69
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Publication Reference
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The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex.
Ziegler SF, Levin SD, Johnson L, Copeland NG, Gilbert DJ, Jenkins NA, Baker E, Sutherland GR, Feldhaus AL, Ramsdell F.
Journal of Immunology 1994 Feb; 152(3):1228.
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Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation.
Yokoyama WM, Maxfield SR, Shevach EM.
Immunological Reviews 1989 Jun; 109:153.
Application：Flow Cyt, Mouse, Mouse T cells.
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Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation.
Yokoyama WM, Koning F, Kehn PJ, Pereira GM, Stingl G, Coligan JE, Shevach EM.
Journal of Immunology 1988 Jul; 141(2):369.
Application：Flow Cyt, Mouse , Mouse lymphocytes.
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The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex.
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