Sell monoclonal antibody, clone MEL-14 (PE)
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More Files
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Flow Cytometry
BALB/c splenocytes were double-stained with Sell monoclonal antibody, clone MEL-14 (PE) (Cat # MAB5737) and rat anti-mouse Cd3e-FITC. Lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Sell.
Immunogen
C3H/eb cloned mouse B lymphoma 38C-13.
Host
Rat
Reactivity
Mouse
Specificity
CD62L/L-selectin, Mr 90 KDa.
Form
Liquid
Conjugation
PE
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c splenocytes were double-stained with Sell monoclonal antibody, clone MEL-14 (PE) (Cat # MAB5737) and rat anti-mouse Cd3e-FITC. Lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Sell
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Publication Reference
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The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.
Siegelman MH, Cheng IC, Weissman IL, Wakeland EK.
Cell 1990 May; 61(4):611.
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Phenotypic analysis of thymocytes that express homing receptors for peripheral lymph nodes.
Reichert RA, Weissman IL, Butcher EC.
Journal of Immunology 1986 May; 136(10):3521.
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A cell-surface molecule involved in organ-specific homing of lymphocytes.
Gallatin WM, Weissman IL, Butcher EC.
Nature 1983 Jul; 304(5921):30.
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The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.
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