Ptprc monoclonal antibody, clone RA3-6B2 (APC/CY7)
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More Files
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Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Ptprc monoclonal antibody, clone RA3-6B2 (FITC) (Cat # MAB5720) and Cd3e monoclonal antibody, clone C363.29B (FITC) (Cat # MAB6100). Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Ptprc.
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells.
Host
Rat
Reactivity
Mouse
Specificity
B220 isoform of CD45, Mr 240 KDa.
Form
Liquid
Conjugation
APC/Cy7
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Ptprc monoclonal antibody, clone RA3-6B2 (FITC) (Cat # MAB5720) and Cd3e monoclonal antibody, clone C363.29B (FITC) (Cat # MAB6100). Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Ptprc
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Interactome
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Publication Reference
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Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells.
Watanabe Y, Akaike T.
Scandinavian Journal of Immunology 1994 May; 39(5):419.
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Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow.
Hardy RR, Carmack CE, Shinton SA, Kemp JD, Hayakawa K.
The Journal of Experimental Medicine 1991 May; 173(5):1213.
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Lymphokine-activated killer (LAK) cells. IV. Characterization of murine LAK effector subpopulations.
Ballas ZK, Rasmussen W.
Journal of Immunology 1990 Jan; 144(1):386.
Application：Flow Cyt, Mouse, Mouse splenocytes.
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Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells.
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