Cd40lg monoclonal antibody, clone MR1 (PE)
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More Files
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Flow Cytometry
Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or plate-bound Cd3e monoclonal antibody, clone 145-2C11 for 7 hours at 37°C. The cells were then harvested, double-stained with Cd40lg monoclonal antibody, clone MR1 (FITC) (Cat # MAB5717) and anti-CD4+8-R-PE, and analyzed by flow cytometry.
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Specification
Product Description
Hamster monoclonal antibody raised against Cd40lg.
Immunogen
Activated mouse Th1 clone D1.6.
Host
Hamster
Reactivity
Mouse
Specificity
CD154 (Mouse CD40 ligand/gp39), Mr 39-KDa.
Form
Liquid
Conjugation
PE
Isotype
IgG, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or plate-bound Cd3e monoclonal antibody, clone 145-2C11 for 7 hours at 37°C. The cells were then harvested, double-stained with Cd40lg monoclonal antibody, clone MR1 (FITC) (Cat # MAB5717) and anti-CD4+8-R-PE, and analyzed by flow cytometry. -
Gene Info — Cd40lg
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Publication Reference
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The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.
Aruffo A, Farrington M, Hollenbaugh D, Li X, Milatovich A, Nonoyama S, Bajorath J, Grosmaire LS, Stenkamp R, Neubauer M, et al..
Cell 1993 Jan; 72(2):291.
Application：Flow Cyt, Human, Monkey, COS cells, Human T cells.
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The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.
Hollenbaugh D, Grosmaire LS, Kullas CD, Chalupny NJ, Braesch-Andersen S, Noelle RJ, Stamenkovic I, Ledbetter JA, Aruffo A.
The EMBO Journal 1992 Dec; 11(12):4313.
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A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells.
Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A.
PNAS 1992 Jul; 89(14):6550.
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The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.
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