Pecam1 monoclonal antibody, clone 390 (APC)
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Specification
Product Description
Rat monoclonal antibody raised against native Pecam1.
Immunogen
Native purified Pecam1 from mouse leukocyte cell line 32D
Host
Rat
Reactivity
Mouse
Specificity
Mouse CD31/PECAM-1, Mr 130-140 KDa.
Form
Liquid
Conjugation
APC
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c splenocytes were first incubated with Pecam1 monoclonal antibody, clone 390 (Cat # MB3230) and then with goatanti-rat Ig-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Pecam1
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Interactome
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Publication Reference
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Platelet endothelial cell adhesion molecule (CD31).
DeLisser HM, Newman PJ, Albelda SM.
Current Topics in Microbiology and Immunology 1993 Jan; 184:37.
Application:Flow Cyt, Human, Human endothelial cells, Human granulocytes, Human monocytes, Human platelets.
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Platelet endothelial cell adhesion molecule (CD31).
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