Il2ra monoclonal antibody, clone 7D4 (SPRD)
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More Files
- More Functions
Flow Cytometry
Cells from BALB/c spleen were activated by PMA (10 ng/mL) and ionomycin (400 ng/mL) for 48 hours, then stained with Il2ra monoclonal antibody, clone 7D4 (FITC) (Cat # MAB5703) and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Il2ra.
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2.
Host
Rat
Reactivity
Mouse
Specificity
Low affinity alpha chain IL-2 receptor (IL-2Ralpha), Mr 55 KDa.
Form
Liquid
Conjugation
SPRD
Isotype
IgM, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Cells from BALB/c spleen were activated by PMA (10 ng/mL) and ionomycin (400 ng/mL) for 48 hours, then stained with Il2ra monoclonal antibody, clone 7D4 (FITC) (Cat # MAB5703) and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Il2ra
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Publication Reference
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Molecular cloning and expression of cDNAs for the human interleukin-2 receptor.
Leonard WJ, Depper JM, Crabtree GR, Rudikoff S, Pumphrey J, Robb RJ, Kronke M, Svetlik PB, Peffer NJ, Waldmann TA, et al..
Nature 1984 Oct; 311(5987):626.
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The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells.
Ortega G, Robb RJ, Shevach EM, Malek TR.
Journal of Immunology 1984 Oct; 133(4):1970.
Application：Flow Cyt, Func, Mouse, HT2 cells.
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Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex.
Malek TR, Robb RJ, Shevach EM.
PNAS 1983 Sep; 80(18):5694.
Application：Flow Cyt, Mouse, Mouse lymphocytes.
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Molecular cloning and expression of cDNAs for the human interleukin-2 receptor.
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