Dpp4 monoclonal antibody, clone 5E8
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Specification
Product Description
Mouse monoclonal antibody raised against native Dpp4.
Immunogen
Native purified rat liver membrane extracts Dpp4.
Host
Mouse
Reactivity
Rat
Specificity
Dipeptidyl peptidase IV (DPP IV) is widely distributed in a number of mammalian tissues and is suggested to play an important role in various kinds of biological processes. DPP IV (CD26) is a serine-type protease that removes the amino-terminal dipeptide from peptide substrate provided that the penultimate amino acid residue is proline or alanine. DPP IV plays an important role in the reclamation of peptide nitrogen from larger peptides. The monoclonal antibody 5E8 reacts with DPP IV present on the apical surface of epithelial cells in the pancreas, small intestine, colon, and bile duct. Furthermore antibody 5E8 reacts with DPP IV on the laminar portions of the proximal renal tubule cells, and, weakly, on the glomeruli.
Form
Liquid
Isotype
IgG1
Recommend Usage
Recommend dilution at 1:10
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% BSA, 0.02% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Dpp4
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Publication Reference
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Identification of a target antigen recognized by a mouse monoclonal antibody to the bile canalicular surface of rat hepatocytes with a random phage display library.
Ariyoshi M, Mizuno M, Morisue Y, Shimada M, Fujita S, Nasu J, Okada H, Shimomura H, Yamamoto K, Tsuji T.
Acta Medica Okayama 2002 Aug; 56(4):187.
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Distribution of asialoglycoprotein receptor in human hepatocellular carcinoma.
Hyodo I, Mizuno M, Yamada G, Tsuji T.
Liver 1993 Apr; 13(2):80.
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Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes.
Mizuno M, Yamada G, Sugiyama T, Vierling JM, Brown WR.
Liver 1987 Oct; 7(5):251.
Application：IHC, WB-Ti, Rat, Liver.
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Identification of a target antigen recognized by a mouse monoclonal antibody to the bile canalicular surface of rat hepatocytes with a random phage display library.
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