Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with LITAF monoclonal antibody, clone AT1F9 (Cat # MAB5642) (1 : 2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppresor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
LPS-induced TNF-alpha factor,lipopolysaccharide-induced TNF-alpha factor,lipopolysaccharide-induced tumor necrosis factor-alpha factor,p53-induced gene 7 protein,small integral membrane protein of lysosome/late endosome,tumor protein p53 inducible protein