IGF1R monoclonal antibody, clone 3C8B1
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using IGF1R monoclonal antibody, clone 3C8B1 (Cat # MAB5588) against truncated IGF1R recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human normal and tumor tissue, showing membrane location using IGF1R monoclonal antibody, clone 3C8B1 (Cat # MAB5588) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant IGF1R.
Immunogen
Recombinant His fusion protein corresponding to IGF1R.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:2000)
Immunohistochemistry (Frozen sections) (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using IGF1R monoclonal antibody, clone 3C8B1 (Cat # MAB5588) against truncated IGF1R recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human normal and tumor tissue, showing membrane location using IGF1R monoclonal antibody, clone 3C8B1 (Cat # MAB5588) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects.
Scheidegger KJ, Cenni B, Picard D, Delafontaine P.
The Journal of Biological Chemistry 2000 Dec; 275(49):38921.
Application：WB-Ce, Rat, RASMCs.
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The contradictions of the insulin-like growth factor 1 receptor.
Baserga R.
Oncogene 2000 Nov; 19(49):5574.
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Structure and function of the type 1 insulin-like growth factor receptor.
Adams TE, Epa VC, Garrett TP, Ward CW.
Cellular and Molecular Life Sciences 2000 Jul; 57(7):1050.
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Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects.
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