GSK3A monoclonal antibody, clone 6G2F1, 6G2F2
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GSK3A.
Immunogen
Recombinant protein corresponding to GSK3A.
Host
Mouse
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:2000)
Immunohistochemistry (Frozen sections) (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using GSK3A monoclonal antibody, clone 6G2F1, 6G2F2 (Cat # MAB5585) against truncated GSK3A recombinant protein (Lane 1) and A-549 (Lane 2) cell lysates.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A) and skin carcinoma (B) tissue, showing cytoplasmical localization, using GSK3A monoclonal antibody, clone 6G2F1, 6G2F2 (Cat # MAB5585) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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GSK-3 is activated by the tyrosine kinase Pyk2 during LPA1-mediated neurite retraction.
Sayas CL, Ariaens A, Ponsioen B, Moolenaar WH.
Molecular Biology of the Cell 2006 Feb; 17(4):1834.
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GSK-3: tricks of the trade for a multi-tasking kinase.
Doble BW, Woodgett JR.
Journal of Cell Science 2003 Apr; 116(Pt 7):1175.
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GSK-3 is activated by the tyrosine kinase Pyk2 during LPA1-mediated neurite retraction.
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