FOXP3 monoclonal antibody, clone 2A11G9, 2A11C2
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More Files
- More Functions
Western Blot
Western blot analysis using FOXP3 monoclonal antibody, clone 2A11G9, 2A11C2 (Cat # MAB5583) against human truncated FOXP3 recombinant protein (Lane 1) and HEK293 cell lysate (Lane 2).
Immunocytochemistry
Immunocytochemical analysis of paraffin-embedded human lymphocyte showing cytoplasmic/nuclear localization, using FOXP3 monoclonal antibody, clone 2A11G9, 2A11C2 (Cat # MAB5583) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FOXP3.
Immunogen
Recombinant protein corresponding to FOXP3.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (1:200-1:1000)
Immunohistochemistry (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using FOXP3 monoclonal antibody, clone 2A11G9, 2A11C2 (Cat # MAB5583) against human truncated FOXP3 recombinant protein (Lane 1) and HEK293 cell lysate (Lane 2).Immunohistochemistry
Immunocytochemistry
Immunocytochemical analysis of paraffin-embedded human lymphocyte showing cytoplasmic/nuclear localization, using FOXP3 monoclonal antibody, clone 2A11G9, 2A11C2 (Cat # MAB5583) with DAB staining.Flow Cytometry
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Publication Reference
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Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level.
Roncador G, Brown PJ, Maestre L, Hue S, Martinez-Torrecuadrada JL, Ling KL, Pratap S, Toms C, Fox BC, Cerundolo V, Powrie F, Banham AH.
European Journal of Immunology 2005 Jun; 35(6):1681.
Application：Flow Cyt, IF, IHC-Fr, IHC-P, WB, Human, Lymph node, Spleen, T cells, Thymus, Tonsil.
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Identifying Foxp3-expressing suppressor T cells with a bicistronic reporter.
Wan YY, Flavell RA.
PNAS 2005 Apr; 102(14):5126.
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Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level.
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