ELK1 monoclonal antibody, clone 7E10D5, 7E10E8
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ELK1.
Immunogen
Recombinant protein corresponding to ELK1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using ELK1 monoclonal antibody, clone 7E10D5, 7E10E8 (Cat # MAB5580) against truncated recombinant ELK1 (Lane 1) and K-562 cell lysate (Lane 2).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells.
Hsieh YH, Wu TT, Tsai JH, Huang CY, Hsieh YS, Liu JY.
Biochemical and Biophysical Research Communications 2006 Jan; 339(1):217.
Application:ChIP, Human, HA22T/VGH cells.
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ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation.
Gille H, Kortenjann M, Thomae O, Moomaw C, Slaughter C, Cobb MH, Shaw PE.
The EMBO Journal 1995 Mar; 14(5):951.
Application:Func, WB-Re, Monkey, COS cells, Recombinant proteins.
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elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints.
Rao VN, Huebner K, Isobe M, ar-Rushdi A, Croce CM, Reddy ES.
Science 1989 Apr; 244(4900):66.
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PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells.
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