DNM2 monoclonal antibody, clone 5E4C2F3
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More Files
- More Functions
Western Blot
Western blot analysis using DNM2 monoclonal antibody, clone 5E4C2F3 (Cat # MAB5579) against truncated DNM2 recombinant protein (Lane 1), SK-N-SH (Lane 2) and NIH/3T3 (Lane 3) cell lysates.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cerebrum (A) and myelencephalon (B) tissue, showing cytoplasmical localization, using DNM2 monoclonal antibody, clone 5E4C2F3 (Cat # MAB5579) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant DNM2.
Immunogen
Recombinant protein corresponding to DNM2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:2000)
Immunohistochemistry (Frozen sections) (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Crude ascites
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis using DNM2 monoclonal antibody, clone 5E4C2F3 (Cat # MAB5579) against truncated DNM2 recombinant protein (Lane 1), SK-N-SH (Lane 2) and NIH/3T3 (Lane 3) cell lysates.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cerebrum (A) and myelencephalon (B) tissue, showing cytoplasmical localization, using DNM2 monoclonal antibody, clone 5E4C2F3 (Cat # MAB5579) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Rapid purification of native dynamin I and colorimetric GTPase assay.
Quan A, Robinson PJ.
Methods in Enzymology 2005 Jan; 404:556.
Application：IP, WB, Sheep, Sheep brains.
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Activation of dynamin I gene expression by Sp1 and Sp3 is required for neuronal differentiation of N1E-115 cells.
Yoo J, Jeong MJ, Kwon BM, Hur MW, Park YM, Han MY.
The Journal of Biological Chemistry 2002 Apr; 277(14):11904.
Application：IF, Mouse, N1E-115 cells.
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Rapid purification of native dynamin I and colorimetric GTPase assay.
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