CHEK1 monoclonal antibody, clone 2G11D5
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant CHEK1.
Immunogen
Recombinant protein corresponding to CHEK1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CHEK1 monoclonal antibody, clone 2G11D5 (Cat # MAB5576) against NIH/3T3 cell lysate.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.
Matsuoka S, Huang M, Elledge SJ.
Science 1998 Dec; 282(5395):1893.
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Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation.
O'Connell MJ, Raleigh JM, Verkade HM, Nurse P.
The EMBO Journal 1997 Feb; 16(3):545.
Application:WB-Tr, WB-Re, Insect, Yeast, Recombinant protein, Sf21 cells, Yeast cells.
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Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.
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