Calcyclin monoclonal antibody, clone 7D11A8
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Calcyclin.
Immunogen
Recombinant GSTs Resin fusion protein corresponding to Calcyclin.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:100-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:500)
Immunohistochemistry (Frozen sections) (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using Calcyclin monoclonal antibody, clone 7D11A8 (Cat # MAB5570) against truncated Calcyclin recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain glioma tissue, showing nuclear localization, using Calcyclin monoclonal antibody, clone 7D11A8 (Cat # MAB5570) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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The Ankrd2, Cdkn1c and calcyclin genes are under the control of MyoD during myogenic differentiation.
Bean C, Salamon M, Raffaello A, Campanaro S, Pallavicini A, Lanfranchi G.
Journal of Molecular Biology 2005 Jun; 349(2):349.
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Calcyclin, a Ca2+ ion-binding protein, contributes to the anabolic effects of simvastatin on bone.
Hwang R, Lee EJ, Kim MH, Li SZ, Jin YJ, Rhee Y, Kim YM, Lim SK.
The Journal of Biological Chemistry 2004 May; 279(20):21239.
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The Ankrd2, Cdkn1c and calcyclin genes are under the control of MyoD during myogenic differentiation.
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