AKT2 monoclonal antibody, clone 1G8C12
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More Files
- More Functions
Western Blot
Western blot analysis using AKT2 monoclonal antibody, clone 1G8C12 (Cat # MAB5563) against truncated AKT2 recombinant protein (Lane 1) and A-431 cell lysate (Lane 2).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant AKT2.
Immunogen
Recombinant protein corresponding to AKT2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
Western Blot (1:100-1:500)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using AKT2 monoclonal antibody, clone 1G8C12 (Cat # MAB5563) against truncated AKT2 recombinant protein (Lane 1) and A-431 cell lysate (Lane 2).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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AKT2 inhibition of cisplatin-induced JNK/p38 and Bax activation by phosphorylation of ASK1: implication of AKT2 in chemoresistance.
Yuan ZQ, Feldman RI, Sussman GE, Coppola D, Nicosia SV, Cheng JQ.
Journal of Biological Chemistry 2003 Jun; 278(26):23432.
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Positive feedback regulation between Akt2 and MyoD during muscle differentiation. Cloning of Akt2 promoter.
Kaneko S, Feldman RI, Yu L, Wu Z, Gritsko T, Shelley SA, Nicosia SV, Nobori T, Cheng JQ.
The Journal of Biological Chemistry 2016 Oct; 291(43):22850.
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AKT2 inhibition of cisplatin-induced JNK/p38 and Bax activation by phosphorylation of ASK1: implication of AKT2 in chemoresistance.
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