MB monoclonal antibody, clone 6H8B5, 5A2G8, 4D7H3, 3H6A7
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using MB monoclonal antibody, clone 6H8B5,5A2G8,4D7H3,3H6A7 (Cat # MAB5539) against truncated MB recombinant protein (GST-tag).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue, showing nuclear and cytoplasmic localization using MB monoclonal antibody, clone 6H8B5, 5A2G8, 4D7H3, 3H6A7 (Cat # MAB5539) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MB.
Immunogen
Recombinant protein corresponding to human MB.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:2000)
Immunohistochemistry (Frozen sections) (1:500-1:2000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using MB monoclonal antibody, clone 6H8B5,5A2G8,4D7H3,3H6A7 (Cat # MAB5539) against truncated MB recombinant protein (GST-tag).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue, showing nuclear and cytoplasmic localization using MB monoclonal antibody, clone 6H8B5, 5A2G8, 4D7H3, 3H6A7 (Cat # MAB5539) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MB
Entrez GeneID
4151Gene Name
MB
Gene Alias
MGC13548, PVALB
Gene Description
myoglobin
Omim ID
160000Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Lack of myoglobin causes a switch in cardiac substrate selection.
Flogel U, Laussmann T, Godecke A, Abanador N, Schafers M, Fingas CD, Metzger S, Levkau B, Jacoby C, Schrader J.
Circulation Research 2005 Apr; 96(8):e68.
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Myoglobin: an essential hemoprotein in striated muscle.
Ordway GA, Garry DJ.
The Journal of Experimental Biology 2004 Sep; 207(Pt 20):3441.
Application：IHC, WB, Human, Mouse, C2C12 cells, Skeletal muscles.
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Lack of myoglobin causes a switch in cardiac substrate selection.
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