Mouse monoclonal antibody raised against recombinant MSH2.
Recombinant protein corresponding to human MSH2.
Western Blot (1:200-1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000) Immunohistochemistry (Frozen sections) (1:200-1:1000) ELISA (1:10000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (50% glycerol, 0.01% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human rectum carcinoma tissue, showing nuclear and cytoplasmic localization using MSH2 monoclonal antibody, clone 3A2B8C (Cat # MAB5538) with DAB staining.
MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. [provided by RefSeq