LCK monoclonal antibody, clone 8E5F9, 4B2C7
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More Files
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Western Blot
Western blot analysis using LCK monoclonal antibody, clone 8E5F9, 4B2C7 (Cat # MAB5528) against truncated LCK recombinant protein (Lane 1) and Jurkat cell lysate (Lane 2).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant LCK.
Immunogen
Recombinant protein corresponding to human LCK.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
Western Blot (1:500-1:2000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis using LCK monoclonal antibody, clone 8E5F9, 4B2C7 (Cat # MAB5528) against truncated LCK recombinant protein (Lane 1) and Jurkat cell lysate (Lane 2).Enzyme-linked Immunoabsorbent Assay
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Gene Info — LCK
Entrez GeneID
3932Gene Name
LCK
Gene Alias
YT16, p56lck, pp58lck
Gene Description
lymphocyte-specific protein tyrosine kinase
Omim ID
153390Gene Ontology
HyperlinkGene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
Other Designations
T-lymphocyte specific protein tyrosine kinase p56lck|p56(LSTRA) protein-tyrosine kinase|protein tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK
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Interactome
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Pathway
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Disease
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Publication Reference
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A constitutively active Lck kinase promotes cell proliferation and resistance to apoptosis through signal transducer and activator of transcription 5b activation.
Shi M, Cooper JC, Yu CL.
Molecular Cancer Research: MCR 2006 Jan; 4(1):39.
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A-770041, a novel and selective small-molecule inhibitor of Lck, prevents heart allograft rejection.
Stachlewitz RF, Hart MA, Bettencourt B, Kebede T, Schwartz A, Ratnofsky SE, Calderwood DJ, Waegell WO, Hirst GC.
The Journal of Pharmacology and Experimental Therapeutics 2005 Oct; 315(1):36.
Application：KA, Recombinant protein.
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The Lck SH3 domain negatively regulates localization to lipid rafts through an interaction with c-Cbl.
Hawash IY, Kesavan KP, Magee AI, Geahlen RL, Harrison ML.
J Biol Chem 2001 Dec; 277(7):5683.
Application：IP, WB-Tr, Human, J.CaM1, Jurkat cells.
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A constitutively active Lck kinase promotes cell proliferation and resistance to apoptosis through signal transducer and activator of transcription 5b activation.
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