Mouse monoclonal antibody raised against full length recombinant FGF2.
Recombinant protein corresponding to full length human FGF2.
The anti-human bFGF antibody reacts with recombinant protein and cell lysate at various concentrations. Its cross reactivity with mouse, rat and other species is not tested. Approx 17 KDa band was observed in recombinant protein and cell lysates(A431, human colon carcinoma tissue or COLO 320DM).
ELISA (1:10000) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Store at 4°C. For long term storage store at -20°C or lower. After reconstitution with deionized water to concentration of 1 mg/mL, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq