Strip blots of crude rat spinal cord extract stained with NEFH monoclonal antibody, clone 9B12 (Cat # MAB5387). All three antibodies bind to a prominent band with an apparent SDS-PAGE molecular weight of 200 KDa.
Mixed neuron/glial cultures stained with NEFH monoclonal antibody, clone 9B12 (Cat # MAB5387) (red) and rabbit GFAP antibody (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue).