GFAP monoclonal antibody, clone 5C10
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat spinal cord extracts stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384). A prominent band at about 50 kDa corresponds to the major isoform of GFAP.
Immunofluorescence
Mixed neuron-glial cultures stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384), our monoclonal antibody to GFAP (red) and chicken polylclonal antibody to neurofilament NF-L (green).
The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes.
The blue channel shows the localization of DNA.
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Specification
Product Description
Mouse monoclonal antibody raised against GFAP.
Immunogen
Purified porcine spinal cord GFAP.
Host
Mouse
Theoretical MW (kDa)
50
Reactivity
Mammals, Pig
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunocytochemistry (1:1000)
Immunofluorescence (1:1000)
Immunohistochemistry (1:1000)
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 5 mM sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot of rat spinal cord extracts stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384). A prominent band at about 50 kDa corresponds to the major isoform of GFAP.Immunohistochemistry
Immunofluorescence
Mixed neuron-glial cultures stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384), our monoclonal antibody to GFAP (red) and chicken polylclonal antibody to neurofilament NF-L (green).
The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes.
The blue channel shows the localization of DNA. -
Gene Info — GFAP
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Publication Reference
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Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A.
Nature Genetics 2001 Jan; 27(1):117.
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Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence.
Bignami A, Eng LF, Dahl D, Uyeda CT.
Brain Research 1972 Aug; 43(2):429.
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Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
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