SNCA monoclonal antibody, clone 2A7
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More Files
- More Functions
Western Blot
Western Blot of SNCA monoclonal antibody, clone 2A7 (Cat # MAB5383) on recombinant SNCA (left lane) and crude extract of mouse brain (right lane, courtesy Rogan Tinsley, Howard Florey Institute, University of Melbourne).
Immunofluorescence
Mixed neuron-glial cultures stained with SNCA monoclonal antibody, clone 2A7 (Cat # MAB5383), our monoclonal antibody to SNCA (red) and chicken polylclonal antibody to MAP2 (green).
The SNCA monoclonal antibody stains vesicular structures the perikarya and processes of the neurons in this image.
Note that some of the neuronal perikarya contain much more alpha-synuclein than others.
The blue channel shows the localization of DNA.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SNCA.
Immunogen
Recombinant His fusion protein corresponding to human SNCA.
Host
Mouse
Theoretical MW (kDa)
15
Reactivity
Human, Mammals
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:10000)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (5 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot of SNCA monoclonal antibody, clone 2A7 (Cat # MAB5383) on recombinant SNCA (left lane) and crude extract of mouse brain (right lane, courtesy Rogan Tinsley, Howard Florey Institute, University of Melbourne).Immunohistochemistry
Immunofluorescence
Mixed neuron-glial cultures stained with SNCA monoclonal antibody, clone 2A7 (Cat # MAB5383), our monoclonal antibody to SNCA (red) and chicken polylclonal antibody to MAP2 (green).
The SNCA monoclonal antibody stains vesicular structures the perikarya and processes of the neurons in this image.
Note that some of the neuronal perikarya contain much more alpha-synuclein than others.
The blue channel shows the localization of DNA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SNCA
Entrez GeneID
6622Protein Accession#
L08850Gene Name
SNCA
Gene Alias
MGC110988, NACP, PARK1, PARK4, PD1
Gene Description
synuclein, alpha (non A4 component of amyloid precursor)
Gene Ontology
HyperlinkGene Summary
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000161559|OTTHUMP00000161561|alpha synuclein|alpha-synuclein|alpha-synuclein, isoform NACP140|non A-beta component of AD amyloid|non A4 component of amyloid
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Interactome
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Disease
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Publication Reference
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The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology.
Julia Obergasteiger, Anne-Marie Castonguay, Sara Pizzi, Stefano Magnabosco, Giulia Frapporti, Evy Lobbestael, Veerle Baekelandt, Andrew A Hicks, Peter P Pramstaller, Claude Gravel, Corrado Corti, Martin Lévesque, Mattia Volta.
NPJ Parkinson's Disease 2023 Mar; 9(1):44.
Application：Co-IP, WB-Ce, Human, Mouse, Mouse substantia nigra pars compacta (SNc), SH-SY5Y cells.
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Increased Levels of the Parkinson's Disease-Associated Gene ITPKB Correlate with Higher Expression Levels of α-Synuclein, Independent of Mutation Status.
Francesca Di Leva, Michele Filosi, Lisa Oyston, Erica Silvestri, Anne Picard, Alexandros A Lavdas, Evy Lobbestael, Veerle Baekelandt, G Gregory Neely, Peter P Pramstaller, Andrew A Hicks, Corrado Corti.
International Journal of Molecular Sciences 2023 Jan; 24(3):1984.
Application：IF, WB-Ce, Human, SK-N-SH cells.
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p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1.
So-Yoon Won, Jung-Jin Park, Soon-Tae You, Jong-A Hyeun, Hyong-Kyu Kim, Byung Kwan Jin, Catriona McLean, Eun-Young Shin, Eung-Gook Kim.
Cell Death & Disease 2022 Jun; 13(6):575.
Application：IP, WB-Ce, WB-Tr, Human, Human brain, SHSY-5Y cells.
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Elevated levels of alpha-synuclein blunt cellular signal transduction downstream of Gq protein-coupled receptors.
Volta M, Lavdas AA, Obergasteiger J, Überbacher C, Picard A, Pramstaller PP, Hicks AA, Corti C.
Cellular Signalling 2016 Nov; 30:82.
Application：IF, IP-WB, Human, CHO, SKN-SH cells.
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alpha-Synuclein and neuronal cell death.
Cookson MR.
Molecular Neurodegeneration 2009 Feb; 4:9.
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Alpha-synuclein locus duplication as a cause of familial Parkinson's disease.
Chartier-Harlin MC, Kachergus J, Roumier C, Mouroux V, Douay X, Lincoln S, Levecque C, Larvor L, Andrieux J, Hulihan M, Waucquier N, Defebvre L, Amouyel P, Farrer M, Destee A.
Lancet 2004 Sep; 364(9440):1167.
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Causal relation between alpha-synuclein gene duplication and familial Parkinson's disease.
Ibanez P, Bonnet AM, Debarges B, Lohmann E, Tison F, Pollak P, Agid Y, Durr A, Brice A.
Lancet 2004 Sep; 364(9440):1169.
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The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology.
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