CSNK1A1 monoclonal antibody, clone AT2E2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate were resolved by SDS-PAGE, transferred to PVDF membrane and probed with CSNK1A1 monoclonal antibody, clone AT2E2 (1:1000).
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CSNK1A1 monoclonal antibody, clone AT2E2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant CSNK1A1.
Immunogen
Recombinant protein corresponding to full length human CSNK1A1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate were resolved by SDS-PAGE, transferred to PVDF membrane and probed with CSNK1A1 monoclonal antibody, clone AT2E2 (1:1000).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CSNK1A1 monoclonal antibody, clone AT2E2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CSNK1A1
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Interactome
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Pathway
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Disease
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Publication Reference
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WNT signaling pathway and stem cell signaling network.
Katoh M, Katoh M.
Clinical Cancer Research 2007 Jul; 13(14):4042.
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The role of the casein kinase 1 (CK1) family in different signaling pathways linked to cancer development.
Knippschild U, Wolff S, Giamas G, Brockschmidt C, Wittau M, Wurl PU, Eismann T, Stoter M.
Onkologie 2005 Oct; 28(10):508.
Application：IHC, WB, Human, Human cancers, Mammalian cells.
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WNT signaling pathway and stem cell signaling network.
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