Western blot analysis of recombinant protein GFP (20, 40 and 80 ng) were resolved by SDS-PAGE, transferred to NC membrane and probed with GFP monoclonal antibody, clone AT2G5 (Cat # MAB5377) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : GFP 20 ng. Lane 2 : GFP 40 ng. Lane 3 : GFP 80 ng.