GFP monoclonal antibody, clone AT2G5
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of recombinant protein GFP (20, 40 and 80 ng) were resolved by SDS-PAGE, transferred to NC membrane and probed with GFP monoclonal antibody, clone AT2G5 (Cat # MAB5377) (1 : 1000).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : GFP 20 ng.
Lane 2 : GFP 40 ng.
Lane 3 : GFP 80 ng.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GFP.
Immunogen
Recombinant protein corresponding to full length Aequorea Victoria GFP.
Host
Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of recombinant protein GFP (20, 40 and 80 ng) were resolved by SDS-PAGE, transferred to NC membrane and probed with GFP monoclonal antibody, clone AT2G5 (Cat # MAB5377) (1 : 1000).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : GFP 20 ng.
Lane 2 : GFP 40 ng.
Lane 3 : GFP 80 ng.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Green fluorescent protein--a bright idea for the study of bacterial protein localization.
Phillips GJ.
FEMS Microbiology Letters 2001 Oct; 204(1):9.
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The green fluorescent protein.
Tsien RY.
Annual Review of Biochemistry 1998 Jan; 67:509.
Application：WB-Tr, Human, Mammalian cells.
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Primary structure of the Aequorea victoria green-fluorescent protein.
Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ.
Gene 1992 Feb; 111(2):229.
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Green fluorescent protein--a bright idea for the study of bacterial protein localization.
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