Western blot analysis of mouse brain extracts (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with GAS7 monoclonal antibody, clone AT4H8 (Cat # MAB5376) (1 : 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described. [provided by RefSeq