This antibody recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. MON 8074 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. This antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. This antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
Immunohistochemistry (Frozen sections) (1:1000-1: 5000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000) Flow Cytometry (1:50-1:100) The optimal working dilution should be determined by the end user.
In PBS (0.09% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq