Aqp1 monoclonal antibody, clone 1/A5F6
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Aqp1.
Immunogen
A synthetic peptide corresponding to amino acids 249-269 of rat Aqp1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
The monoclonal antibody recognizes an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28). Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and the kidney. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Frozen sections) (1:500-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
ELISA (1:1000-1:20000)
Immunoprecipitation (1:1000-1:10000)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide, 0.1% BSA)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Aqp1
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Publication Reference
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Posttraumatic invasion of monocytes across the blood-cerebrospinal fluid barrier.
Szmydynger-Chodobska J, Strazielle N, Gandy JR, Keefe TH, Zink BJ, Ghersi-Egea JF, Chodobski A.
Journal of Cerebral Blood Flow and Metabolism 2012 Jan; 32(1):93.
Application:IF, Rat, Choroidal epithelial cells.
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Posttraumatic invasion of monocytes across the blood-cerebrospinal fluid barrier.
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