Mouse monoclonal antibody raised against synthetic peptide of Citrullinated Fibrinogen.
A synthetic peptide corresponding to mouse Citrullinated Fibrinogen.
Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deiminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis. Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get deiminated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline.
The optimal working dilution should be determined by the end user.
Lyophilized from 10 mM ammonium bicarbonate buffer
Store at 4°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.