CD47 monoclonal antibody, clone BRIC126
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Specification
Product Description
Mouse monoclonal antibody raised against native purified CD47.
Immunogen
Native purified CD47 from human erythrocytes.
Host
Mouse
Reactivity
Bovine, Dog, Human, Pig
Specificity
This antibody recognizes the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.
Form
Liquid
Isotype
IgG2b
Recommend Usage
Flow Cytometry (10 ul of the suggested working dilution to label 106 cells in 100 ul)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycerine, pH 7.8 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
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Gene Info — CD47
Entrez GeneID
961Gene Name
CD47
Gene Alias
IAP, MER6, OA3
Gene Description
CD47 molecule
Omim ID
601028Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD47 antigen|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associa
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Interactome
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Pathway
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