Rat monoclonal antibody raised against IL10RA/IL10RB.
This antibody recognizes human CD210, a 90-110kD glycoprotein, also known as Interleukin-10 receptor (IL-10R). CD210 is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low. Recent work has reported an increase in intracellular expression of CD210 during dendritic cell maturation, but a reduction in surface CD210 expression (3). This clone is reported to function as a blocking antibody. Due to low expression of CD210, sensitive staining techniques may be required.
Flow Cytometry (10ul of the suggested working dilution (1:10-1:50) to label 1x 106 cells in 100ul) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq
The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21. [provided by RefSeq
cytokine receptor class-II CRF2-4,cytokine receptor family II, member 4