Rat Tumors, epithelial origin monoclonal antibody, clone CC52
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Specification
Product Description
Mouse monoclonal antibody raised against Rat Tumors, epithelial origin.
Immunogen
Native purified from rat colon adenocarcinoma cells (CC531).
Host
Mouse
Reactivity
Rat
Specificity
CC52 is a rat strain-independent marker for tumor cells of epithelial origin, such as colon, breast, or lung cancer, etc. When injected in colon tumor-bearing rats, CC52 localized to tumor cells (Hagenaars et al., 2001). CC52 can be used for immunohistochemical staining of tumor cells in frozen tissue sections as well as formalin-fixed/paraffin-embedded tissue. CC52 is also suitable for flow cytometry, Western blotting, immunoprecipitation, and ELISA. Hybridomas were selected for producing antibodies reacting with the CC531 cell line and with minimal cross reactivity to normal tissues (Hagenaars et al., 2001). CC52 belongs to a first generation of such antibodies and is suitable to detect tumor cells by immunohistochemistry. The monoclonal antibody binds to a dimer of two proteins, 120 kD, 130 kD, expressed by rat tumor cells of epithelial origin. CC52 has been used in many studies in a rat model of colorectal cancer (Hagenaars et al., 2000). In immunohistochemical stainings, there is
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In buffer containing 0.05% sodium azide
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
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