Mouse monoclonal antibody raised against synthetic peptide MUC1.
A synthetic peptide (conjugated with BSA) corresponding to 60mer tandem repeat of MUC1.
VU-5F12 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largly consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of VU-5F12 is as established with "epitope fingerprinting". Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by subcloning four times.
The optimal working dilution should be determined by the end user.
In PBS (0.05% sodium azide)
Store at 4°C.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.