Ly6c1/Ly6c2 monoclonal antibody, clone ER-MP20
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Specification
Product Description
Rat monoclonal antibody raised against native Ly6c1/Ly6c2.
Immunogen
Native purified Ly6c1/Ly6c2 from mouse macrophage cell lines.
Host
Rat
Reactivity
Mouse
Specificity
The monoclonal antibody ER-MP20 specifically reacts with mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes). The antigen is a 14 kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. The monoclonal antibody ER-MP20 has been raised after immunization of rats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells. The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti-F4/80) is recommended.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Recommend dilution at 1:50
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% BSA, 0.02% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Detection Antibody
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Gene Info — Ly6c1
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Gene Info — LOC100041546
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