Cytokeratin monoclonal antibody, clone C-11 (APC)
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Specification
Product Description
Mouse monoclonal antibody raised against native cytokeratin.
Immunogen
Native purified cytokeratin from Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Host
Mouse
Reactivity
Human, Mammals
Specificity
This antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Form
Liquid
Conjugation
APC
Concentration
0.1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
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Publication Reference
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Peptide-based isolation of circulating tumor cells by magnetic nanoparticles.
Bai L, Du Y, Peng J, Liu Y, Wang Y, Yang Y, Wang C.
Journal of Materials Chemistry B 2014 Apr; 2:4080.
Application:IF-CTC, Human, Blood.
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New Generation of Ensemble-Decision Aliquot Ranking Based on Simplified Microfluidic Components for Large-Capacity Trapping of Circulating Tumor Cells.
Zhao M, Nelson WC, Wei B, Schiro PG, Hakimi BM, Johnson ES, Anand RK, Gyurkey GS, White LM, Whiting SH, Coveler AL, Chiu DT.
Analytical Chemistry 2013 Oct; 85(20):9671.
Application:Conjugation, Human, Circulating Tumor Cells.
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Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats.
Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER.
Kidney International 2005 Dec; 68(6):2572.
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Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis.
Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T.
Res Commun Mol Pathol Pharmacol 1998 Aug; 101(2):115.
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A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, Kovarík J.
The Journal of Pathology 1991 Jul; 164(3):215.
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Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread.
Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J.
International Journal of Cancer. Supplement 1988 Jan; 3:50.
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Peptide-based isolation of circulating tumor cells by magnetic nanoparticles.
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