Cd4 monoclonal antibody, clone GK1.5 (APC)
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Specification
Product Description
Rat monoclonal antibody raised against native Cd4.
Immunogen
Mouse CTL clone V4 cells.
Host
Rat
Theoretical MW (kDa)
55
Reactivity
Mouse
Specificity
This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 KDa).
Form
Liquid
Conjugation
APC
Concentration
0.1 mg/mL
Isotype
IgG2b
Recommend Usage
Flow Cytometry (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
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Gene Info — Cd4
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Publication Reference
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Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells.
Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI.
Journal of Immunology 2008 May; 180(10):6593.
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Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice.
Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y.
Transplantation 2008 Apr; 85(8):1167.
Application:Flow Cyt, Func, Mouse, Mouse T cells.
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Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes.
Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM.
Nature Immunology 2007 Apr; 8(4):388.
Application:Func, Mouse, EK35 cells.
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T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1.
Zheng B, Han S, Kelsoe G.
The Journal of Experimental Medicine 1996 Sep; 184(3):1083.
Application:Flow Cyt, Mouse, Mouse T cells.
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Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia.
Gavett SH, Chen X, Finkelman F, Wills-Karp M.
American Journal of Respiratory Cell and Molecular Biology 1994 Jun; 10(6):587.
Application:Flow Cyt, Mouse, Mouse T cells.
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Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells.
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