CD86 monoclonal antibody, clone BU63 (PerCP)
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Specification
Product Description
Mouse monoclonal antibody raised against native CD86.
Immunogen
Native purified CD86 from B-lymphoblastoid cell line ARH 77.
Host
Mouse
Theoretical MW (kDa)
70
Reactivity
Human
Specificity
This antibody reacts with CD86 (B7-2), a 70 KDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Form
Liquid
Conjugation
PerCP
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Surface staining of human peripheral blood cells with CD86 monoclonal antibody, clone BU63 (PerCP) (Cat # MAB5112). -
Gene Info — CD86
Entrez GeneID
942Gene Name
CD86
Gene Alias
B7-2, B70, CD28LG2, LAB72, MGC34413
Gene Description
CD86 molecule
Omim ID
601020Gene Ontology
HyperlinkGene Summary
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
Other Designations
B-lymphocyte activation antigen B7-2|B7-2 antigen|CD28 antigen ligand 2|CD86 antigen|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2
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Interactome
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Pathway
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Publication Reference
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Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.
Giguere JF, Bounou S, Paquette JS, Madrenas J, Tremblay MJ.
Journal of Virology 2004 Jun; 78(12):6222.
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Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules.
Mauri D, Wyss-Coray T, Gallati H, Pichler WJ.
Journal of Immunology 1995 Jul; 155(1):118.
Application:Func, Human, Antigen-Presenting T Cells.
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B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells.
Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J.
The Journal of Experimental Medicine 1994 Nov; 180(5):1841.
Application:Flow Cyt, Human, Human dendritic cells.
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The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.
Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF.
Blood 1992 Jun; 69(6):915.
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Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.
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