CD59 monoclonal antibody, clone MEM-43 (PE)
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Flow Cytometry
Surface staining of human peripheral blood cells with CD59 monoclonal antibody, clone MEM-43 (PE) (Cat # MAB5011).
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Specification
Product Description
Mouse monoclonal antibody raised against native CD59.
Immunogen
Native purified CD59 from thymocytes and T lymphocytes.
Host
Mouse
Theoretical MW (kDa)
18-20
Reactivity
Human
Specificity
This antibody reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 KDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.
Form
Liquid
Conjugation
PE
Isotype
IgG2a
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Surface staining of human peripheral blood cells with CD59 monoclonal antibody, clone MEM-43 (PE) (Cat # MAB5011). -
Gene Info — CD59
Entrez GeneID
966Gene Name
CD59
Gene Alias
16.3A5, 1F5, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20
Gene Description
CD59 molecule, complement regulatory protein
Omim ID
107271Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
20 kDa homologous restriction factor|CD59 antigen|CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 glycoprotein|Ly-6-like protein|T cell-activating protein|human leukocyte antigen MIC11|lymphocytic a
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity.
Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP.
Journal of Immunology 2006 Mar; 176(5):2915.
Application：Flow Cyt, Human, K-562 cells.
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Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.
Cebecauer M, Cerny J, Horejsi V.
Biochemical and Biophysical Research Communications 1998 Feb; 243(3):706.
Application：Flow Cyt, Monkey, COS-7 cells.
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Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59.
Bodian DL, Davis SJ, Morgan BP, Rushmere NK.
The Journal of Experimental Medicine 1997 Feb; 185(3):507.
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Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity.
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