Mouse monoclonal antibody raised against 5-methyl Cytosine.
Ovalbumine conjugated with 5-methyl Cytosine.
ELISA (0.1-0.3 ug/mL) Dot Blot (1-2 ug/mL) Immunofluorescence (1:100) Flow Cytometry (1-2 ug/mL) Methylated DNA Immunoprecipitation (1-5 ug/IP) The optimal working dilution should be determined by the end user.
Aliquot and store at -80°C. Aliquot to avoid repeated freezing and thawing.
Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952). A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um. B. Enlarged picture corresponding to a region from the left panel (as indicated in A). C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B).
MeDIP assay was performed using fragmented genomic DNA from the MCF7 breast cancer cells, 5-mC monoclonal antibody, clone b (Cat # MAB4952) and optimized PCR primer sets for qPCR of the indicated regions. 1 ug of antibody was used per IP experiment. The hypermethylated p14 INK4A intronic CpG island and Alu repeats are clearly recovered, whereas the non-methylated promoter-CpG island of the p14 INK4A gene is not (red bars). One IP negative control (no antibody added) was included in the assay, the background is extremely low (white bars).