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Last updated: 2016/10/23
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5-mC monoclonal antibody, clone b

  • Catalog # : MAB4952
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against 5-methyl Cytosine.
  • Immunogen:
  • Ovalbumine conjugated with 5-methyl Cytosine.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (0.1-0.3 ug/mL)
    Dot Blot (1-2 ug/mL)
    Immunofluorescence (1:100)
    Flow Cytometry (1-2 ug/mL)
    Methylated DNA Immunoprecipitation (1-5 ug/IP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS
  • Storage Instruction:
  • Aliquot and store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Indirect immunofluorescence results obtained with 5-mC monoclonal antibody, clone b (Cat # MAB4952).
    A. HeLa cells were immunofluorescent labelled with 5-mC monoclonal antibody, clone b (Cat # MAB4952) is diluted 1 : 100 followed by a goat anti-mouse FITC conjugated antibody. Scale bar is 75 um.
    B. Enlarged picture corresponding to a region from the left panel (as indicated in A).
    C. Nuclei were DAPI stained to label specifically the DNA (same region as shown in the middle panel : B).
  • Immunoprecipitation
  • Immunoprecipitation
  • MeDIP assay was performed using fragmented genomic DNA from the MCF7 breast cancer cells, 5-mC monoclonal antibody, clone b (Cat # MAB4952) and optimized PCR primer sets for qPCR of the indicated regions.
    1 ug of antibody was used per IP experiment.
    The hypermethylated p14 INK4A intronic CpG island and Alu repeats are clearly recovered, whereas the non-methylated promoter-CpG island of the p14 INK4A gene is not (red bars).
    One IP negative control (no antibody added) was included in the assay, the background is extremely low (white bars).
  • Dot Blot
  • Methylated DNA Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Dot Blot
  • Methylated DNA Immunoprecipitation
  • Flow Cytometry
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