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Last updated: 2017/3/19
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H3K27me2/3 monoclonal antibody

  • Catalog # : MAB4947
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against H3K27me2/3.
  • Immunogen:
  • The region of the histone H3 containing the methylated lysine 27 (H3K27me2/3).
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • Dot Blot (1:300)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot was performed using histone extracts from HeLa cells (HeLa HE, 20 ug) and H3K27me2/3 monoclonal antibody (Cat # MAB4947) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. The location of the protein of interest is indicated with an arrow.
  • Immunofluorescence
  • Dot Blot
  • Dot Blot
  • Dot Blot is performed to determine the cross-reactivity of H3K27me2/3 monoclonal antibody (Cat # MAB4947) with other histone modifications.
    Other histone modifications include mono-methylation of lysine 27 and mono-, di- and trimethylation of two other lysines of H3 (K9 and K36).
    To determine the cross-reactivity, 0.2 to 100 pmol of peptide containing the respective histone modifications are spotted on a membrane.
    The antibody is used at a dilution of 1 : 300. The antibody recognizes di- and trimethylation of lysine 27 on histone H3.
  • Application Image
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
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