Native purified CD29 from U937 myelomonocytic cells.
The molecular weight of the recognized antigen is 130KDa. CD29 is the integrin B1 chain which is expressed as heterodimer non-covalently associated with the integrin alpha subunits 1 to 6 (CD49a to CD49f) and known as very late antigens (VLA). It is also called platelets GPlla. It has been reported as receptor of fibronectin. The antigen mediates cellular adhesion. It has a broad cellular reactivity but is not expressed by erythrocytes. In normal peripheral blood CD29 is found on approximately 41% of T cells, 40% of CD4+ T lymphocytes, 43% of CD8+ T lymphocytes (1). It is slightly reactive with peripheral B-cells (1). The coexpression of CD4 and CD29 allows the identification of the helper/inducer subpopulation of CD4 lymphocytes. Clone Lia1/2 is a blocking antibody.
Immunofluorescence (2ug/5x105 cells) Flow Cytometry (2ug/5x105 cells/test) Immunohistochemistry (1:25-1:50) The optimal working dilution should be determined by the end user.
Lyophilized from PBS (1mg/mL BSA)
Store at 4°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.