CD48 monoclonal antibody, clone MEM-102 (APC)
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Specification
Product Description
Mouse monoclonal antibody raised against native CD48.
Immunogen
Native purified CD48 from Raji Burkitt's lymphoma cell line.
Host
Mouse
Theoretical MW (kDa)
40-47
Reactivity
Human, Non-Human Primates
Specificity
This antibody reacts with CD48 antigen (Blast-1), a 40-47 KDa GPI-anchored membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells; it is negative on granulocytes, platelets and erythrocytes.
Form
Liquid
Conjugation
APC
Isotype
IgG1
Recommend Usage
Flow Cytometry (10 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
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Gene Info — CD48
Entrez GeneID
962Gene Name
CD48
Gene Alias
BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48
Gene Description
CD48 molecule
Omim ID
109530Gene Ontology
HyperlinkGene Summary
BLAST1 is the designation used for an activation-associated cell surface glycoprotein of 40 to 45 kD expressed primarily in mitogen-stimulated human lymphocytes. The protein sequence predicted by the cDNA encoding BLAST1 indicates that BLAST1 is a member of the immunoglobulin supergene family. Yokoyama (1991) identified the BLAST1 activation/adhesion molecule as CD48.[supplied by OMIM
Other Designations
CD48 antigen (B-cell membrane protein)|OTTHUMP00000025680|OTTHUMP00000060268
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Publication Reference
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CD48 is an allergen and IL-3-induced activation molecule on eosinophils.
Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F.
Journal of Immunology 2006 Jul; 177(1):77.
Application：Flow Cyt, Human, Mouse, Human nasal polyp eosinophils, Peripheral blood eosinophils, Mouse bronchoalveolar lavage fluid.
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The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1.
Korínek V, Stefanová I, Angelisová P, Hilgert I, Horejsí V.
Immunogenetics 1991 Jan; 33(2):108.
Application：WB-Ce, WB-Tr, Human, Monkey, COS, PBMCs, Raji cells.
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Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.
Bazil V, Stefanová I, Hilgert I, Kristofová H, Vanĕk S, Bukovský A, Horejsí V.
Folia Biol (Praha) 1989 Jan; 35(5):289.
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CD48 is an allergen and IL-3-induced activation molecule on eosinophils.
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